Abstract: Selection of some strains is considered the first step in improving mango production. So, this study was done for a three successive seasons (2003 – 2005) on two Egyptian mango strains "Hania" and "Aml" to describe them, horticulturally and identification those genetically utilizing DNA fingerprint.
The vegetative characters (leaf shape, length, width, etc) and histological characteristics (number of stomata per mm (stomatal density), stomata length and width) showed great variation between the two studied strains.
The fruit weight was 581 gm for Hania strain and 1020 gm for Aml strain. The two strains fruits had good characters as shape, net weight ratio, firmness, SSC, TA, Vit. C and total sugars.
In generally, physical and chemical properties of Aml strain fruits were better than Hania strain.
These differences of horticultural aspects due to genetic variances, which were determined by using SSR markers, of the 42 primers screened, 36 primers gave reproducible polymorphic DNA amplification patterns.
60.7 % of the scored fragments are considered putative genotypes-specific markers in both strains. The polymorphic
information content (PIC values) ranged from 0.25 to 0.75, with a mean value 0.51 for all loci.
The heterozygosity level was 0.68 and 0.53 for Hania and Aml strains, respectively. By banding patterns obtained from these 36
primers, each strain in this study could be distinguished from the other, indicating that, PCR by using SSR primers was an efficient method for genotype identification.
[M. T. Wahdan, A. Z. Abdelsalam, A. A. El-Naggar and M. A. Hussein. Preliminary Horticultural Studies To Describe And Identify Of Two New Egyptian Mango Strains Using DNA Fingerprint. Journal of American Science 2011; 7(2):641-650].
primers, each strain in this study could be distinguished from the other, indicating that, PCR by using SSR primers was an efficient method for genotype identification.
[M. T. Wahdan, A. Z. Abdelsalam, A. A. El-Naggar and M. A. Hussein. Preliminary Horticultural Studies To Describe And Identify Of Two New Egyptian Mango Strains Using DNA Fingerprint. Journal of American Science 2011; 7(2):641-650].
(ISSN: 1545-1003). http://www.americanscience.org.
Key words: Mango, Histological, Morphological, fruit, DNA, Fingerprint, SSR
Key words: Mango, Histological, Morphological, fruit, DNA, Fingerprint, SSR
5. Conclusion
Two mango strains "Hania" and "Aml" differed in
vegetative characters (leaf shape, length, width, etc)
and histological characteristics (number of stomata
per mm (stomatal density), stomata length and
width).
vegetative characters (leaf shape, length, width, etc)
and histological characteristics (number of stomata
per mm (stomatal density), stomata length and
width).
It can be concluded that these two new strains
had a good fruit properties.
had a good fruit properties.
The fruit weight was 581 gm for Hania strain and 1020 gm for Aml strain.
The two strains fruits had good characters as shape, net weight ratio, firmness, SSC, TA, Vit. C and total sugars.
In generally, physical and chemical properties
of Aml strain fruits were better than Hania strain.
of Aml strain fruits were better than Hania strain.
These differences of horticultural aspects due to
genetic variances, which were determined by using
SSR markers, of the 42 primers screened, 36 primers
gave reproducible polymorphic DNA amplification
patterns. 60.7 % of the scored fragments are
considered putative genotypes-specific markers in
both strains.
Corresponding Author
genetic variances, which were determined by using
SSR markers, of the 42 primers screened, 36 primers
gave reproducible polymorphic DNA amplification
patterns. 60.7 % of the scored fragments are
considered putative genotypes-specific markers in
both strains.
Corresponding Author
M. T. Wahdan
Horti. Dept. Fac. of Agric., Suez Canal University,
Ismailia Governorate, Egypt.
Box. P. 41522
Wahdan2011@gmail.com
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